| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
27/12/2000 |
|
Data da última atualização: |
27/12/2000 |
|
Autoria: |
SALLES, J. F.; GITAHY, P. de M.; SKOT, L.; BALDANI, J. I. |
|
Título: |
Use of endophytic diazotrophic bacteria as a vector to express the cry3A from Bacillus thuringiensis. |
|
Ano de publicação: |
2000 |
|
Fonte/Imprenta: |
Brazilian Journal of Microbiology, Brasilia, v. 31, p. 155-161, 2000. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southem-blot using an intemal fragment of 1.0 kb as a probe. The production of 8-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Westem-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The 8-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the 8-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
|
|
Palavras-Chave: |
Bactéria diazotrófica endofítica; Endophytic diazotrophy bacteria. |
|
Thesagro: |
Bacillus Thuringiensis. |
|
Thesaurus Nal: |
Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02046naa a2200217 a 4500
001 1597290
005 2000-12-27
008 2000 bl --- 0-- u #d
100 1 $aSALLES, J. F.
245 $aUse of endophytic diazotrophic bacteria as a vector to express the cry3A from Bacillus thuringiensis.
260 $c2000
520 $aThe goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southem-blot using an intemal fragment of 1.0 kb as a probe. The production of 8-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Westem-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The 8-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the 8-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
650 $aGluconacetobacter diazotrophicus
650 $aHerbaspirillum seropedicae
650 $aBacillus Thuringiensis
653 $aBactéria diazotrófica endofítica
653 $aEndophytic diazotrophy bacteria
700 1 $aGITAHY, P. de M.
700 1 $aSKOT, L.
700 1 $aBALDANI, J. I.
773 $tBrazilian Journal of Microbiology, Brasilia$gv. 31, p. 155-161, 2000.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 8 | |
| 5. |  | GUIMARÃES, C. M.; STONE, L. F.; CASTRO, A. P. de; BORGES, M. G.; CUNHA, D. G. M. Desempenho de genótipos de arroz de terras altas irrigados por aspersão, sob alta temperatura natural. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 9., 2015, Pelotas. Ciência e tecnologia para otimização da orizicultura: anais. Brasília, DF: Embrapa; Pelotas: Sosbai, 2015. 1 pen-drive.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
|    |
| 6. | | GUIMARÃES, C. M.; MORAES, O. P. de; CASTRO, A. P. de; STONE, L. F.; BORGES, M. G.; CUNHA, D. G. M. Avaliação da população de seleção recorrente CG136 para tolerância à deficiência hídrica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
| |
| 7. | | CUNHA, D. G. M.; GUIMARÃES, C. M.; MELO, L. C.; BORGES, M. G.; LUCAS, J. de F.; SOUZA, M. G. de; TEIXEIRA, A. G. R.; PERES, B. A. Avaliação de famílias F5:8 do cruzamento 'BRS Pérola' x BAT 477 sob deficiência hídrica e irrigação adequada. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
| |
| 8. | | BORGES, M. G.; GUIMARAES, C. M.; MELO, L. C.; CUNHA, D. G. M.; SILVA NETO, J. B. da; SOUZA, R. O.; SOUZA, R. O. Desenvolvimento radicular de famílias F5:8 de feijão do cruzamento 'BRS Pérola' X BAT 477 com e sem deficiência hídrica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
| |
| Registros recuperados : 8 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
